You have now completed the first "flush" (round) of mushroom growth.The goal here is go slowly so that your water absorbs into the growing medium versus pooling up at the bottom. If using a 3lbs bag:add 80 mL of water back into your bag. If using a 5lbs bag: add 100 mL of water back into your bag. Rehydrate your growing medium by spraying distilled water or RO water down the sides of the bag.Set the mushrooms aside on a clean plate/tray/bin/etc. Lightly twist and pull at the base of the stem to remove the mushrooms. Open the bag and harvest ALL the mushrooms.It's best to begin harvesting when most of the mushrooms are ready.ĭuring the harvesting stage, be sure to handle the bag gently to prevent the spores from dropping. There may be mushrooms at different stages. The best time to harvest is after the caps have formed and just before the veil tears away. Observe your bag’s progression and watch for signs of fruiting (actual mushroom growth). If Using Spores: Store your bag for about 2-4 weeks. If Using Liquid Culture: Store your bag for about 1-3 weeks. You will store you bag until it is 20-40% colonized (20-40% covered in white mycelium).Pro Tip: keeping your environment at 75-78 degrees Fahrenheit will result in faster rates of colonization. Store the bag in a semi-dark room with no direct sunlight, off the ground, at about 70-78 degrees Fahrenheit.If you’re doing multiple injections with the same needle, make sure to re-sterilize your needle with a lighter between each injection.Cover the injection port with clean scotch tape to protect the bag from outside contamination. You may also place the alcohol wipe back on the injection port and allow the injection port to self-heal.Dispose of the needle in a designated receptacle. Carefully pull the needle out the injection port and place the cap safely back on. If you’re working with spores, make sure that all the fluid is injected in a single spot in the bag so the reproductive cells are near each other. Carefully insert your syringe needle into the injection port and inject the bag with 10-20 CCs of liquid culture or spores.Flame sterilize your syringe needle and allow it to cool. Attach the sterile needle to your syringe.
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